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KMID : 1123920080220030580
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 3 p.580 ~ p.584
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Protective Effect of Radix Clematidis Extract on Streptozotocin-induced Diabetes
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Ham Kyung-Wan
Kim Eun-Kyung
Song Mi-Young
Kwon Kang-Beom
Song Je-Ho
Seo Eun-A
Ryu Do-Gon
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Abstract
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In the present study, Radix clematidis extract (RCE) was evaluated to determine if it could protect pancreatic cells against multiple low dose streptozotocin (MLDS)-induced diabetes. Injection of mice with MLDS resulted in hyperglycemia and hypoinsulinemia, which was confirmed by immunohistochemical staining. However, the induction of diabetes by MLDS was completely prevented when mice were pre-administrated with RCE. Generation of oxidative stress is implicated in MLDS, a cell specific toxin-induced islet cell death. In this context, to elucidate the mechanisms of protective effects in RCE pre-administrated diabetic mice, we investigated the expression of heme oxygenase-1 (HO-1), which is one of the anti-oxidant enzymes. MLDS-induced HO-1 expressions were significantly reduced in MLDS-treated mice. However, the decrease of HO-1 by MLDS were protected by pretreatment of RCE. The molecular mechanism by which RCE inhibits diabetic conditions by MLDS appears to involve inhibition of HO-1 expression. Taken together, these results reveal the possible therapeutic value of RCE for the prevention of type 1 diabetes progression.
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KEYWORD
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Radix clematidis, diabetes, multiple low-dose streptozotocin, heme oxygenase-1
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